Glucose Uptake Assay Kit (Colorimetric) from Abcam

Product Specs
ItemGlucose Uptake Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab136955
Quantity100 test
Sample TypeAdherent cells, Suspension cells
Sensitivity0.01 nmol/well
Time3h 0m
ApplicationsCellular Assay
ReactivityMammals, Mammals, Other species
TypeCell-based (quantitative)
TargetGlucose uptake

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Antitumor Effects of PRMT5 Inhibition in SarcomasCancer Research CommunicationsNovember 2, 2023Stéphanie Verbeke, Aurélien Bourdon, Jean-Philippe Guegan, Laura Leroy, Vanessa Chaire, Elodie Richard, Alban Bessede, Antoine ItalianoTo confirm the role of PRMT5 in glycolysis, we used a glucose uptake assay kit (Abcam, catalog no. ab136955) and a lactate colorimetric assay kit (Sigma-Aldrich, catalog no. MAK065) according to the protocols

(2) Knockdown of SETD5 inhibited glycolysis and tumor growth in gastric cancer cells by down-regulating Akt signaling pathwayOpen Life SciencesOctober 24, 2023Jing Shi, Litao Yu, Changhong Zhu, Haiyan Zhongintake test The glycolysis levels of cells were detected according to the glucose intake kit (Abcam, ab136955) according to the relevant instructions. 2.9 Oxygen consumption rate (OCR) and extracellular acidification

(3) Endoplasmic Reticulum Stress Promotes the Expression of TNF-α in THP-1 Cells by Mechanisms Involving ROS/CHOP/HIF-1α and MAPK/NF-κB PathwaysInternational Journal of Molecular SciencesOctober 14, 2023Nadeem Akhter, Ajit Wilson, Hossein Arefanian, Reeby Thomas, Shihab Kochumon, Fatema Al-Rashed, Mohamed Abu-Farha, Ashraf Al-Madhoun, Fahd Al-Mulla, Rasheed Ahmad, et al.(5% CO2) for 18 h. Glucose uptake assay was performed, following the manufacturer’s protocol (Cat. #ab136955, Colorimetric glucose uptake assay kit; Abcam, MA, USA). Briefly, cells were washed 3 times and split

(4) N6-methyladenosine methyltransferase KIAA1429 promoted ovarian cancer aerobic glycolysis and progression through enhancing ENO1 expressionBiology DirectOctober 9, 2023Lijuan Gan, Shengchao Zhao, Yang Gao, Yuwen Qi, Min Su, Anjin Wang, Hongbing Caiproduction Glucose consumption was measured by the glucose uptake assay following the manufacturer's instructions (ab136955, Abcam, USA). After treatment with 2-DG, cells were placed in a 37 °C incubator for 20 min, followed

(5) Long noncoding RNA DLEU1 promotes proliferation and glycolysis of gastric cancer cells via APOC1 upregulation by recruiting SMYD2 to induce trimethylation of H3K4 modificationTranslational OncologyOctober 1, 2023Haitao Xu, Zhichang Ba, Chunxun Liu, Xuefeng YuBuckinghamshire, UK). Determination of glucose uptake capacity A glucose uptake colorimetric determination kit (ab136955, Abcam) was employed to determine glucose uptake as per the instructions of the manufacturer. In a word,

(6) Patulin Ameliorates Hypertrophied Lipid Accumulation and Lipopolysaccharide-Induced Inflammatory Response by Modulating Mitochondrial RespirationAntioxidants (Basel, Switzerland)September 11, 2023Hong S, Park SK, Lee J, Park SH, Kim YS, Park JH, Yu S, Lee YG.Measurement of Glycolipid Metabolism Glucose concentration was measured using a glucose assay kit (#ab136955, Abcam). Briefly, the amount of glucose released into the culture medium by the cells was measured by

(7) Identification of novel genes associated with exercise and calorie restriction effects in skeletal muscleAging (Albany NY)June 12, 2023Jae Sook Kang, Min Ju Kim, Eun-Soo Kwon, Kwang-Pyo Lee, Chuna Kim, Ki-Sun Kwon, Yong Ryoul Yangmyotubes transfected with esiRNA for each target gene using a glucose uptake assay kit from Abcam (#ab136955) according to manufacturer’s instructions. C2C12 myotubes were differentiated using 2% FBS DMEM and

(8) The Muscle-Conditioned Medium Containing Protocatechuic Acid Improves Insulin Resistance by Modulating Muscle Communication with Liver and Adipose TissueInternational Journal of Molecular SciencesMay 30, 2023Hira Shakoor, Haneen Hussein, Nour Al-Hassan, Mouza Alketbi, Jaleel Kizhakkayil, Carine Platatwas assayed according to the established protocol from a commercially available glucose uptake kit (ab136955; Abcam, Cambridge, UK) in C2C12 myotubes, 3T3-L1 adipocytes, and HepG2. C2C12 cells were seeded in a

(9) METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cellsEMBO reportsApril 5, 2023Hou Y, Zhang X, Yao H, Hou L, Zhang Q, Tao E, Zhu X, Jiang S, Ren Y, Hong X, et al.uptake assay Glucose uptake assay was performed using Glucose Uptake Colorimetric Assay Kit (Abcam, ab136955) according to the manufacturer's instructions. For measurement of glucose uptake, cells were seeded

(10) Antidepression of Xingpijieyu formula targets gut microbiota derived from depressive disorderCNS neuroscience & therapeuticsFebruary 1, 2023Li Y, Yang L, Li J, Gao W, Zhao Z, Dong K, Duan W, Dai B, Guo R.(G1P) level and adenosine triphosphate (ATP) content that were measured by Glucose Uptake Assay Kit (Ab136955, Abcam, Shanghai, China), Glucose‐1‐phosphate assay kit (Ab155892, Abcam, Shanghai, China), ATP Assay

(11) S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironmentHeliyonFebruary 1, 2023Jia-qi Yuan, Shou-man Wang, Lei Guowas detected following the manufacturer’s introductions (glucose uptake assay kit, BioVision/Abcam, ab136955). When measuring lactate production, normal control and S100A9 silencing cells were successively cultured

(12) WDR79 promotes aerobic glycolysis of pancreatic ductal adenocarcinoma (PDAC) by the suppression of SIRT4Open Med (Wars)January 16, 2023Wenke Yin, Xiaoyan Song, Yue XiangGlucose intake test The glycolysis levels of cells were detected according to the glucose intake (ab136955), which was measured by the corresponding kits bought from Abcam according to the relevant instructions. 2.9

(13) Glucose transporter GLUT1 expression is important for oriental river prawn (Macrobrachium nipponense) hemocyte adaptation to hypoxic conditionsJournal of Biological ChemistryJanuary 1, 2023Xichao Sun, Cheng Xue, Yiting Jin, Chao Bian, Na Zhou, Shengming Sun(70). Glucose uptake, lactate production, and ATP generation assays A 2-Deoxyglucose Glucose Uptake Assay Kit (ab136955; Abcam) kit was used to measure glucose uptake. In brief, cells were incubated with the glucose analog

(14) Never-in-Mitosis A-Related Kinase 8 (NEK8) Regulates Adipogenesis, Glucose Homeostasis, and ObesityOxidative Medicine and Cellular LongevityNovember 30, 2022Yan-Jun Wang, Ying Zhao, Yun-Wei Sun, Yan Chenmin. Then, the glucose uptake and lactate product were measured using the Glucose Uptake Assay Kit (ab136955) from Abcam (Shanghai, China) according to the manufacturer's instructions. 2.8. Animals and Groups The

(15) UBR7 inhibits HCC tumorigenesis by targeting Keap1/Nrf2/Bach1/HK2 and glycolysisJournal of Experimental & Clinical Cancer ResearchNovember 24, 2022Liang Zhao, Min Kang, Xiaomeng Liu, Zhenran Wang, Yan Wang, Haiqiang Chen, Wenhui Liu, Shiqian Liu, Baibei Li, Chong Li, et al.software. Lactate Assay, Glucose Uptake Assay and ATP Assay Lactate assay kit (ab65330), Glucose Uptake Assay (ab136955) and ATP assay (ab83355) were purchased from Abcam. The detection of lactate, glucose uptake and ATP

(16) Long noncoding RNA DIO3OS induces glycolytic-dominant metabolic reprogramming to promote aromatase inhibitor resistance in breast cancerNature communicationsNovember 22, 2022Chen X, Luo R, Zhang Y, Ye S, Zeng X, Liu J, Huang D, Liu Y, Liu Q, Luo ML, et al.40% confluence and replenished with fresh medium. Twenty-four hours later, Glucose Uptake Assay Kit (#ab136955, Abcam), Lactate Colorimetric Assay Kit II (#K627-100, BioVision) or PicoProbe™ Acetyl CoA Assay Kit

(17) Development of a novel diagnostic assay for insulin receptor autoantibodies based on a patient with autoimmune hypoglycaemiaFront Endocrinol (Lausanne)October 25, 2022Leiluo Geng, Cheuk-Lik Wong, Boya Liao, Ying Lin, Hao Han, Karen S. L. Lam, Aimin Xu, Chi-Ho Lee, Vicki H. K. Taminsulin for 30 min, followed by measurement of glucose uptake capacity with a commercial kit (Abcam, #ab136955). Immunoblotting analysis Total protein was isolated from cells using RIPA buffer (150 mM NaCl, 1.0%

(18) Hypoxia-Inducible Expression of Annexin A6 Enhances the Resistance of Triple-Negative Breast Cancer Cells to EGFR and AR AntagonistsCellsSeptember 27, 2022Stephen D. Williams, Tunde M. Smith, LaMonica V. Stewart, Amos M. Sakweuptake by the indicated TNBC cell lines was determined using a glucose uptake assay kit (Colorimetric, ab136955, Abcam) as per the manufacturer’s Instructions. The indicated TNBC cell lines were seeded at a density

(19) The MBNL1/circNTRK2/PAX5 pathway regulates aerobic glycolysis in glioblastoma cells by encoding a novel protein NTRK2-243aaCell death & diseaseSeptember 5, 2022Zhao Y, Song J, Dong W, Liu X, Yang C, Wang D, Xue Y, Ruan X, Liu L, Wang P, et al.Sigma-Aldrich, MO, USA) for 30 min. Intracellular glucose levels were detected by glucose uptake test (ab136955, Abcam, USA) according to the instructions. Briefly, cells were lysed using lysis buffer, then heated

(20) Heat Shock Protein 70 Mediates the Protective Effect of Naringenin on High-Glucose-Induced Alterations of Endothelial FunctionInternational Journal of EndocrinologyAugust 1, 2022Zhihan Zhang, Hui Liu, Xiang Hu, Yikang He, Lu Li, Xinyu Yang, Changhua Wang, Mingbai Hu, Shengxiang Taocontrol. 2.3. Determination of Glucose Uptake and PKA Activity Glucose Uptake Assay Kit (Colorimetric, ab136955, Abcam) and PKA Kinase Activity Kit (ab139435, Abcam) were used to measure glucose uptake and PKA activity

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Glucose Uptake Assay Kit (Colorimetric) from Abcam

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Glucose Uptake Assay Kit (Colorimetric) from Abcam

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